Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 3, 2008; Page No.(633-638)

ISOLATION OF A TUERMOSTABLE LECTIN FROM THE LEAVES OF PROCUMBANS LINN

A. P. RAMTEKE AND M. B. PATIL

Abstract

T. procumballs L, a wild medicinal plant was found to contain galactose specific lectin in leaves. The lectin was purified to homogeneity by affinity chromatography and was found to have molecular weight of 23 kD. The purified lectin could agglutinate erythrocytes of human blood group 'A', 'B', '0', rabbit and other animal. Fhe lectin did not require any metal ion for agglutination, however Hg— inhibited the agglutination. The agglutination was also inhibited by a - D— galactose and galactose derivatives. The lectin was found to exhibit a and 13 - galactosidase activities. The lectin was found to be stable at higher temperatures and a wide range of pH.

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