Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 3, 2008; Page No.(675-627)

ANTIBACTERIAL ACTIVITY OF PLINICA GRANATLIM LINN. FRUIT RIND EXTRACTS AND FRACTIONS ON ENTEROPATHOGENIC ESCHERICHIA COLI

S. RAJAN, S. BALAKUMARI AND T. THIRUNALASUNDARI

Abstract

Antibacterial activity of Punica granaturn fruit rind extracts and fractions were studied on Enteropathogenic strains of Eschcrichia coll.. Disc diffusion method (Bauer et al., 1966) was adapted to screen antibacterial activity and agar dilution method (NCCLS, 1993) was used to look for Minimum Inhibitory, Concentration (MIC). Antibacterial activity was observed in two extracts and various fractions. MIC of the hexane and water fraction were found to be 50 ug/mL concentration and methanol, chloroform and benzene fractions were at 100 1...tg/ml. Aqueous extracts taken with ethyl alcohol and water showed MIC at 200 ug/ ml concentrations.

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