Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

CHITINASE PRODUCTION BY SOLID STATE FERMENTATION USING SHRIMP WASTE

Y.L. RAMACHANDRA, S. PADMALATHA RAI, P.S. SUJAN GANAPATHY, H.V. SUDEEP AND N.B. KRISHNAMURTHY

Abstract

In the present work we screened 187 acidophylic chitinolytic microbes and made an attempt to produce chitinase by Solid State Fermentation (SSF) using shrimp waste as substrate for 3 different selected microorganisms. It was observed a maximum of 56.8, 34.0, 17.2 U/gIDS chitinase production by Oerskovia sp., Sporolactobacillus sp. 2, Sporolactobacillus sp.1, respectively after 72 hours of incubation in-a shrimp waste medium containing 60% moisture content and in an arbitrarily selected conditions of 32°C. Results of the present study indicate scope for the utilization of shrimp waste as a novel substrate for chi tinase production by Solid State Fermentation.