Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 3, 2008; Page No.(557-561)

ANTIMICROBIAL POTENTIAL OF ETHANOLIC EXTRACTS OF INDIAN PROPOLIS (EEIP'S) AGAINST HUMAN PATHOGENS

S. MURUGAN, P UMA DEVI, S. SELVI, P. CMNNASWAMY AND K.R. MANI

Abstract

The aim of this study is to evaluate the antimicrobial activity of Indian propolis. The in vitro activity of propolis against Gram positive bacteria Methicillin Resistant Staphylococcus aureus (MRSA), Streptococcus mu tans, Gram negative bacteria include Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella typhi, Salmonella typhimurium, Vibrio cholerae; yeast include Candida albicans, Candida cruezi isolated from human infections was studied by the well diffusion method. Antimicrobial activity was tested with 95% Ethanolic Extracts of Indian Propolis (EEIP's) against the test microorganisms. Among the bacteria, Gram positive MRSA growth was inhibited at 900 1.1g/mL followed by S. mutans, P. aeruginosa, V cholerae, Salmonella typhimurium, S. typhi, K.pneumoniae all being inhibited at 700 i..ig/mL. E. coli was highly susceptible among the tested microorganisms at 600p,g/mL. Among the yeast strains tested, Candida albicans was inhibited -at 7001.1g/mL where as the other strain C.cruzei exhibited high resistance. The relative order of susceptibility ambng the tested microorganisms was: S.aureus > s.mutans = P.aeruginosa = V.cholerae = Salmonella typhimurium = S.typhi = K.pneumoniae = Candida albicans > E.coli. Our findings confirm the traditional therapeutical claims for Indian propolis.

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