Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

ANTIBACTERIAL ACTIVITY OF PLINICA GRANATLIM LINN. FRUIT RIND EXTRACTS FRACTIONS ON ENTEROPATHOGENIC ESCHERICHIA COLI

S. RAJAN, S. BALAKUMAR AND T. THIRUNALASUNDARI

Abstract

Antibacterial activity of Punica granatum fruit rind extracts & fractions were studied on Enteropathogenic strains of Escherichia co/i. Disc diffusion method (Baiter et al- 1966) was adapted to screen antibacterial activity and agar dilution method (NCCLS , 1993) was used to look for Minimum Inhibitory Concentration (MIC). Antibacterial activity was observed in two extracts and various fractions. WC of the hexane & water fraction were found to be 50 ig / ml concentration and methanol, chloroform & benzene fractions were at 100 ig / ml. Aqueous extracts taken with ethyl alcohol and water showed MIC at 200 ig / ml concentrations.