Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 1, 2008; Page No.(29-35)

PURIFICATION AND WASH PERFORMANCE ANALYSIS OF THERMOSTABLE EXTRACELLULAR ALKALINE PROTEASE PRODUCED BY SOIL BACTERIUM BACILLUS SP. GOS-2

R. SELVAKUMAR R. SATHISH KUMAR AND K. SWAMINATHAN

Abstract

The purpose of this work was to study the purification, characterization and industrial application of alkaline protease produced by newly isolated Bacillus sp. 00S-2 from soil samples collected in and around Coimbatore, Tamilnadu, India. The enzyme was purified in a 2-step procedure, involving acetone precipitation and Sephadex 0-100 gel filtration chromatography. The purified enzyme was subjected to SDSPAOE for determining the molecular weight and was found to be 18 KDa. The enzyme had a maximum activity at 60°C and pH 10. The compatibility of the enzyme was studied with surfactant, oxidizing agent, optical brightener and commercial detergents in the absence of stabilizers. Increase in the concentration of the surfactant and oxidizing agent decreased the enzyme activity whereas optical brightener did not have any effect on the enzyme activity. The enzyme was found to retain maximum enzyme activity of 93.84% with Power detergent powder when compared to other detergents. The efficacy of the purified alkaline protease was tested with 1% (w/v) Power detergent on blood stained cloth for its wash performance. The enzyme was effective in removal of 25jim of bloodstain along with Power detergent powder at 55°C. The compatibility and stain removal properties of protease find potential application in detergents industry.

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