Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 10, Issue 1, 2008; Page No.(11-14)

RESTRICTION PROFILE STUDIES OF THE ENVELOPE PROTEIN GENE (P32) OF SHEEP PDXVIRUS ISOLATES FROM INDIA

S. RAJAMUTHU, M. PARTHIBAN, A. WILSON ARUNI AND P. RAMADASS

Abstract

Seven local field isolates of sheep poxvirus from different districts of TamilNadu and two vaccine strains were characterized by PCR-RFLP assay. The viral envelope protein gene P32 of sheep poxvirus was amplified by PCR technique using sheep poxvirus specific primers. The amplified products were subjected to different restriction enzymes digestion, viz. Mbo II, Alu I and Hinf I. The digestion yielded identical restriction patterns and differentiation was found improbable with no variable regions with in the P32 gene.

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