Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 4, 2007; Page No.(1013-1016)

PLASMID PROFILE OF MULTID RUG RESISTANT ESCHERICHIA COLI ISOLATED FROM DIABETIC PATIENTS WITH ASYMPTOMATIC BACTERIURIA

C. DHARANI, A. KASTHURI THILAGAM, S. ARJUNAN AND S. KANNAN

Abstract

Urine samples collected from 60 diabetic patients were screened for asymptomatic bacteriuria. Out of them 30% (18 samples) were culture positive. The uropathogenic isolates include Escherichia coli, Proteits spp and Klebsiella spp. Escherichia coli was the predominant bacterium and caused 25% (15/ 60samples) of infections. Among the 15 Escherichin coil isolates, seven were multidrug resistant. All the multidrug resistants have the plasmid of 23.1Kb. The restriction digestion of the plasmid DNA with the enzymes Kpnl and Noll showed different restriction pattern, which authenticates that the multidrug resistant strains are belonging to three different strains. Among the diabetic patients asymptomatic bacteriuria was more common in women compared to men at the age group of 50-60 years old. The occurrence of multidrug resistant Escherichia coil was prevalent in diabetic men while compared with diabetic women.

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