Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 4, 2007; Page No.(983-988)

ANTIMICROBIAL ACTIVITY OF TAXLIS BACCATA L. (HIMALAYAN YEW) BARK ON CERTAIN CLINICALLY SIGNIFICANT MICROBES

K. TAYUNG

Abstract

Taxus baccata an evergreen gymnosperm plant is an important source of Taxol- a proven anticancer drug. Traditionally the bark of this plant is used for preparing beverages locally known as Nanikin chay and use as medicine in many regions of Himalayas. Antimicrobial activity of Taxus baccata bark was tested by Agar cup diffusion method against some important clinically significant microbes. The solvents used for extraction were acetone, 95% ethanol, methanol and petroleum ether. Dried powdered bark was used for extraction. Acetone extract was found to be potent, out of the four solvents used. The bark extracts was found to be most effective against Shigella flexneri (22mm) and Bacillus subtilis (20mm) and least effective against Candida albicans (10mm). Very less Antimicrobial activity was observed in petroleum ether extract and no activity was detected in 95%ethanol extract. In broth culture, acetone extract had MIC values ranges from 1 000mg-300mg / mL.

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