FLUORIDE INDUCED DNA DAMAGE AND APOPTOSIS IN RAT PANCREASA. SHASHI, NEERAJ SHARMA AND MONIKA BHARDWAJ
The present study was undertaken to find out cytochemical changes in pancreas in experimental fluorosis. Sprague Dawley albino rats of both sexes in the weight range of 100 to 300 gm were used for the experiments. They were divided into 6 experimental groups and one control group. The rats of control group were administered subcutaneously double distilled water as lcc/kg bw daily. The experimental groups of rats were injected with 30, 45 and 75 mg NaF/kg bw/day for 36 days. The experimental period was divided into 2 phases at interval of 15 and 36 days. Animals were sacrificed from each group at the end of 16, and 37 days. Cytochemical examination of pancreas showed uniform distribution of RNA in all cellular component of the pancreas. The exocrine glands exhibited intense localization of DNA in the pancreas of control rat. The level of DNA elevated in acini as well as islets of Langerhans in fluoridated groups of rat. The islets showed proliferation and apoptosis in the pancreas of rats treated with 30, 45 and 75 mg/kg bw /day for 36 days. The atrophied intercalated ducts revealed decrease in localization of RNA in test rats treated with 75 mg NaF/kg bw/day for 36 days.
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