Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 4, 2007; Page No.(949-951)

IN-VITRO ISOLATION AND CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM MURINE BONE MARROW

A.MANGALA GOWRI, A. GNANAMANI AND A. MAHALINGA NAINAR

Abstract

The dearth of transplantable tissues has raised the hope on stem cells ability to replace worn out tissues. The stem cell tissue repair uses the body's own three-dimensional matrix and growth factor milieu. The enormous potential of stem cells for therapy lies mainly on its isolation and characterization of its pluripotency and plasticity. Hence, in this study, the mechanisms for isolating, culturing and characterizing the stem cells from bone marrow of mice, which is the principal model in mammalian studies, have been carried out. The cultured cells were confirmed for their osteogenic potential by its exhibition of alkaline phosphatase activity.

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