RECOMBINANT DNA TECHNOLOGY FOR BIOREMEDIATIONDIPTY SINGH AND M.H. FULEKARAbstract Bioremediation is the recent treatment technology for hazardous wastes generated by the industries. This involves the ability of either indigenous or genetically modified microorganisms to enzymatically degrade the complex organic compounds into environmental friendly substances such as inorganics, carbon dioxide and biomass. The microorganisms are stimulated by supplying electron acceptor/electron donor, nutrients, energy and carbon source from biodegraded compounds. Sometimes the single microorganism may not degrade complex compounds when present in higher concentration. The recombinant DNA technology in 1972 has given birth to new dimension for biodegradation of compounds which are not possible to degrade by indigenous microorganism. Genetically modified microorganism has proved beneficial to biodegrade the large range of complex compounds. The basic concept of recombinant DNA technology, application of genetically modified microorganisms (GM0s) for environmental restoration are discussed in this paper.
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