Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 4, 2007; Page No.(849-854)

CHARACTERIZATION OF K INETIC PARAMETERS OF EXTRACELLULAR TANNASE AND GALLIC ACID PRODUCED ON POMEGRANATE HIND (PUNICA GRANATUM) BY ANISOLATED MOTOR SPECIES

ANITA SRIVASTAVA AND RITA KAR

Abstract

Pomegranate rind (PR) was found to be a potential tannin rich substrate for tannase and gallic acid production by a Mucor species.Optimization of the fermentation parameters showed maximum enzyme and gallic acid production at 37°C, pH 5.0 with 4°/o(w/v) PR and mineral salt solution after 72 h.The enzyme was optimally active at a temperature of 35°C and pH 6.2.The addition of 1% tannic acid stimulated the production by 3.9 fold while 1.5% tannic acid repressed the activity by 69.5%.The Kin values of the enzyme produced on pure tannic acid and PR were determined from Lineweaver Burk plot and found to be 0.022 and 0.019 respectively.

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