Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 4, 2007; Page No.(803-806)

MICROPROPAGATION OF PASSIFLORA FOETIDA L. VAR. HISPIDA (DC,) KILLIP

K. H. SMILA, S. SALMA, S. SHOBANA, S. SANGEETHA, A. RENUGADEVI, K. REVATHI AND M. RAJASEKARA PANDIAN

Abstract

Passiflora foetida L. var Hispida, a Climber belonging to the family Passifloraceae has been micropropagated successfully by culturing nodal and internodal segments. The explants were cultured in MS basal medium augmented with 6 benzylaminopurine. Maximum percentage of shoot proliferation was achieved in MS basal medium fortified with 6 Benzylamino purine (4.44 :M). The maximum percentage of callus formation was achieved on MS medium augmented 1,vith 2, 4-Dichlorophenoxy acetic acid (4.52 :M). Maximum percentage of shoot proliferation from the internodal derived calli was achieved on MS medium supplemented with 6 Benzyl amino purine (2.221.1M and 4.44:M). The in vitro raised shootlets were transferred to MS medium fortified with IBA and highest percentage of rooting was observed in Indole-3- Butyric acid (4.90 :M).

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