STUDIES ON EFFECT OF PYOCYANIN PIGMENT ON PATHOGENS COMMONLY INVOLVED IN PYOGENIC INFECTIONSAYESHA G. SIDDIQUI, U. N. RAMDHAMI, B.D. SHELAKE, M.K. RANJEKAR AND B.S. NAGOBA
An attempt has been made to study the effect of pyocyanin pigment using three different concentrations on common bacterial pathogens involved in pyogenic infections. P. aeruginosa isolated in pure culture from pus was used for production of pyocyanin pigment in King's A broth. The presence of pyocyanin was confirmed by development of bluish green colour and also by spectrophotometric studies. Effect of extracted pyocyanin in three different dilutions and crude pyocyanin was studied against E. coli, Staph. aureus, Proteus vulgaris and Klebsiella sp. Pyocyanin at higher concentration showed inhibitory effects against E.coli, Staph. aureus, Klebsiella sp., but not against Proteus vulgaris. However, at lower concentration, no inhibitory effect was seen with any of the bacterium.
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