Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 3, 2007; Page No.(669-673)

A NOVEL METHOD FOR RAPID CLONAL PROPAGATION OF MUSSAENDAR YTHROPHYLLA `ROSEA' THROUGH TISSUE CULTURE

P.C.THAKUR,AND HARSH KUMAR

Abstract

Different factors that affect culture establishment, axillary shoot proliferation and rooting of microshoots of Mussaenda erythrophylla 'Rosea', a woody ornamental and medicinal shrub, were studied for its clonal micropropagation. Nodal stem cuttings were the most suitable explants for proliferation of multiple shoots when cultured on MS (Murashige and Skoog 1962) basal medium (BM) containing 2.0 mg/ L Kinetin (MN) and 0.5 mg/L- naphthalene acetic acid (NAA) which yielded an average of 4.3 shoots per explant. Less frequency of axillary shoots were prod/uced by shoot tip explants. KIN alone was less effective in shoot proliferation. Browning of medium due to phenolics exedution was checked when explants were treated with ascorbic acid (AA). Best rooting of microshoots was observed on half-strength BM supplemented with NAA (2.0 mg/L) which also developed basal callus. However, basal callus posed little problem in establishment of plantlets to the field. Microshoots were directly differentiated from explants Without going to callus phase which eliminated chances of culture induced genetic variations. Thus, a novel rapid micropropagation protocol was developed that ensured clonal stability and genetic uniformity.

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