Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 9, Issue 3, 2007; Page No.(641-643)

IN VITRO PLAN I I ET REGENERATION OF CUCUMBER (CLICTIMIS SATIVUS L.) USING BIOPRESERVER (PYRAMID)

V. GURUCHANDRAN , M. VA RADARAJ NN, S. M. NAGARAJAN*, V. GURUCHANDRAN , M. VA RADARAJ NN, S. M. NAGARAJAN, C. SUDHA, R.VIDHYA, R. SARANYA, H.N. AYSHA FARVIN, G. RAJESWARI AND S. SURYA

Abstract

A newly designed Biopreserver (pyramid) was employed for in vitro culture of cucumber - using cotyledon explants. High frequency of multiple shoots were noticed in cultures incubated in biopreserver than the cultures incubated in normal incubation room conditions. Maximum number of shoots were formed in MS-medium with 2mg/L of BAP.

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