EFFECT OF GAMMA-IRRADIATION ON LEAF PROTEIN PROFILE OF SESA MUM INDICLIM LSHARATH T. POOJARY, VINAYA MAIITRE AND BEI-INAZ B. PATEL
Sesame ( Sisarnum indicum I.inn.), is an important oil crop of India. It is an important source of edible oil and is widely used as spice and in natural seasoning. Sesame is also used as a synergist for pyrethrum insecticides (Simon et al. 1984) During the Mutation breeding programme using gamma- radiations on the above plant with the objective of producing better mutant varieties with higher oil yields studies were carried out on the effect of gamma-rays on the protein profile of the leaves. Seeds of S indicum L. var Gujarat Til-1 (MT-67-52) were irradiated with different doses of gamma radiations and were sown simultaneously with non-irradiated as control. They were germinated and allowed to grow normally while their growth characteristics were recorded. The plants obtained from irradiated seeds were compared with control plants for apparent phenotypic variations. The leaves were later subjected to quantitative and qualitative Protein analysis using protein estimation and electrophoresis technique along with control as reference/control. It was found that the protein concentration (in mg/gm) of the leaf material decreased progressively with the increase in dose of irradiation. The implication of this decrease in protein concentration may be attributed to gross mutations like deletions caused by gamma radiations, resulting in certain proteins not being expressed. SDS - PAGE analysis showed the presence of new peptides in leaves obtained from seeds exposed to 30Krad and 40 Kraci of irradiation, indicating the de novo synthesis of novel polypeptides at higher dose of irradiation.
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