PROPERTIES OF CLONED ALPHA AMYLASE FROM E.COLIS.M. DHARMADHIKARI, S.A. PESHWE AND M.S. ANDHALE
Bacillus sp. SK2 expressing a amylase gene was purified, it was showing maximum absorption at 259 nm and Tm in between 85 to 90 "C. The a amylase gene from Bacillus sp. SK2 was cloned and expressed in E. coli BJ 83. The transformed E.coli secreted up to 64% of the cloned gene product into the medium. The recombinant enzyme showed improved functionality in terms of activity at a wider pH range and at higher temperature, as compared to the crude enzyme from the donor strain. The cloned E. coli resulted maximum a amylase activity after 24 hrs as compared to Bacillus sp. (48 hrs). The transformed cells produced substantial amylolytic activity. A seven-fold increase in a amylase activity was found in cell suspension as compared to cell free extract.
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