Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

STRESS INDUCED CATALASE ACTIVITY IN VIGNA MUNGO

K. M. REMYA, T. DEBORAH PARIPURANAM, H. SRIDHAR AND S. UMAMAHESWARI

Abstract

Catalase is the antioxidant heme containing redox enzyme present in all mammalian cells. It catalyses the decomposition of hydrogen peroxide (H2O2) to water and oxygen. Catalase protects the cells from toxic compounds such as super oxide radical O2 -.Catalase activity was studied under different days, different photoperiod within a day, under in vitro condition on Murashige and Skoog (MS) medium, under different stress conditions such as UV, water, salt and pesticide. The result suggests that stress induces the catalase activity upon to a certain limit after which it decreases. This decreased activity is due to intolerance of plant in stress condition. Peroxisomes were isolated from the plant sample and their activity was determined. SDS-PAGE technique was used in which a single band was formed indicating that the peroxisomes might be the catalase protein.