CITRIC ACID PRODUCTION FROM SUGARCANE BAGASSE THROUGH SOLID STATE FERMENTATION BY MUTANTS OF ASPERGILLLIS NIGERV. SELVI, K.S. KANNA, R. BANERIEE, G. SINGH AND L.C. RAM
A mutant of Aspergillus niger - COPS (soil isolate) was selected after ultra violet irradiation of the spores for the production of citric acid by Solid State Fermentation from sugar cane bagasse. This mutant was capable of giving comparatively higher yields of citric acid than the wild strain. Citric acid production with mutant strain of A. niger was optimized with different pH and moisture content of the substrate, fermentation time and with different carbon sources. Carbon sources like sucrose, glucose and fructose were studied for the yield of citric acid. The accumulation of citric acid reached the maximum on the 9th day of the incubation. Of the three levels of initial moisture contents viz 60,70 and 80% of bagasse tested, 60% resulted in the highest yield on the 9th day of incubation. The yield under this condition was found to be upto 16.5mg/m1 of bagasse. Best results in terms of citric acid concentration, production, yield were obtained with a substrate pH of 4.
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