Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 4, 2006; Page No.(761-764)

STUDIES ON IN VITRO PRODUCED CALLUS AND SHOOTS OF RAPHANIIS SATIVIIS L.

VIJAY VENUGOPAL AND BEHNAZ B PATEL

Abstract

A series of experiments were carried out in order to optimize a protocol for in vitro shoot regeneration of Radish and to analyze the phenomenon of organogenesis in vitro. White radish (Raphanus sativus L.) was used to analyze the effect of explant source (cotyledons or hypocotyl), culture media and plant growth regulators. Our findings showed that hypocotyl from the seeds germinated in vitro presented lower levels of contamination and a better development than cotyledons. The MS media showed better results than B5 for the cultivar and experimental lines studied. MS media supplemented with appropriate concentration of Indole 3-acetic acid (IAA) and 6-benzylaminopurine (BAP) for shoot regeneration from callus gave consistently better results. Protein estimation of the callus and shoot regenerated in vitro was also performed and was compared with that of matured leaves and roots. In the process a 37 kD protein was found to be expressed (through SDS - PAGE) in callus obtained in vitro but absent in matured leaves or roots.

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