Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 3, 2006; Page No.(557-660)

PRODUCTION OF AMYLASE IN LIQUID CULTURE BY STRAIN OF BACILLUS SUBTILIS

S. P. TBIANDE AND B. K. RAAIAM

Abstract

Various reports have appeared on microbial synthesis of anwiase on solid substrates and in mt\iia under stationary and submerged conditions. It appears from all these reports that the .iuction ot anwlase is dependent on the strains, composition of nk-xlia and methods of cultivation. An -1a.se producing t_vcteria were isolated form soil and cultural conditions were optimized for production yiase. Studies on nutritional requirements of Bacillus s4thtilis for production of ani)ia... revealed that :um conditions were achieved in fermentation culture medium containing 2% starch and incubated at for 4 days at pH 7.0.The strain was found to utilize starch as best carbon source followed by wheat floor for uction of amylase. Peptone was utilized as best nit,7-en source. 0.2% viv was opfimum inoculum size, thsilding accelerated the enzyme production. Maximum enzyme production was found with incubationperkx1 96 hours. Enzyme activity was exhibited at pH 7 and temperature 3r."4k1 NaClaocelerates enzyme activity At lower concentration. M,gC1.2 and CuSQ4also accelerated the enzyme .activity. Mcubation period for optimum enzyme activity was 20 minutes, envane concentration tvas found to be 0,8ml and optimum substrate concentration was 400 microgram in was found be 1428 Rg mos and tucks Min from simple L- B plot, The Mcnaelis - Niemen Constant (Km) and malitniumn maction velocity- (Vmax) were determined by Linewever Burk Plots using starch ( kx-Inc...-_,ntration between 100ugms to 1000 Kgms).

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