Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 8, Issue 3, 2006; Page No.(473-474)

IN VITRO STUDIES ON STATIETENIA MAITAGONI (L.) JACQ.

S.M.NAGARAJAN, S. RAJA SEKARAN, S.KANTDASAMY AND R. JEYA CHANDRAN

Abstract

Effect of plant growth regulators on callus formation and multiple shoot induction was studied in Swietenia mahagoni(t) Jacq. For this MS basal media supplemented with plant growth regulators viz., NAA, 2,4-D and BAP in different combinations were used. Various explants such as petiole, leaf, stern, shoot tip and nodes were taken as standard explants. The best callusing efficiency was observed in all the three explants namely leaf, stern and petiole on basal media supplemented with 2,4-D (3 mg/L) +NAA (0.1 mg/L). The best multiple shoot forming capacity was observed in both shoot tip and axillary bud explants cultured on basal media with BAP (3 mg/L)+NAA (0.1 mg/L).

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