EXTRACTION AND ESTIMATION OF INDO,E-3-ACETIC ACID (IAA) FROM SOME ENDOPHYTIC AND PATHOGENIC COELOMYCETESV. BHUVANESWARI, G. KATHIRAVAN, V. GANGADEVI AND J. MUTHUMARY
Most of Ihe physiological activities and growth in plants are regulated by the action and interaction of some growth regulators like Auxins. Chemically it is Indole - 3 - acetic acid (IAA). Soon after tl1Q, discovery of hidole -3 - acetic acid (IAA) in higher plants, Auxin activity was also detected in fungi. I lowever, IAA biosynthetic pathways were identified in only a few fungi. In the present investigation, Indole - 3 - acetic acid (IAA) was extracted and estimated from three species of Camarosporium (Endophytic) and Collooirichum lidcalum (pathogenic). Auxin generally occurs bound with an aminoacid or sugar. Over six different precursor molecules for Auxins I ia ve been reported. Many workers reported that the aminoacid, 'Tryi)tophan, figures prominently in the Auxin formation. hi the present investigation Auxin production was Tryptopkw dependent. No IAA or any other in dole!: were detected in ihe absence of Tryptophan, while in Ole presence of Tryptophan IAA production was hir)h. IAA production was more in culture filtrates when c("Pantd to the mycelium of the test fungi. Out of the two different species of Coclomycetes tested, the pal I If wtnic non_crU ;pi iyuc fungus, Collelotrichum Mail urt-1 prod ticp( I I nure amount of IAA when compared lo the endophytic.
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