Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol.13, Issue 2, 2011; Page No.(251-256)

PRELIMINARY PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL ACTIVITY OF SPHAERANTHUS INDICUS LINN.

S. AHMED JOHN AND B. TAMIL MARAI SELVI

Abstract

Leaves, stem and roots of S. indicus were extracted separately with methanol, ethanol, chloroform, petroleum ether and hot water and the extracts were screened for its phytochemical constituents. The plant revealed the presence of alkaloids, saponins, tannins, flavonoids, steroids, terpenoids, cardiac glycosides, aminoacids, monosaccharides and reducing sugar. Leaves extracts showed significant amount of phytochemicals and hence antimicrobial studies of leaves extracts were carried out against bacterial species like Bacillus sp, Staphylococcus sp, Klebsiella sp, E. coli, Pseudomonas sp using filter paper and agar well diffusion method at 4 different concentrations. Aqueous extract showed the highest inhibitory effect compared to all other extracts and it showed good inhibitory activity against Bacillus sp followed by Staphylococcus sp. The gram positive bacteria were found to be more susceptible than gram negative bacteria. Antifungal activity of methanol and ethanol extracts were tested against Penicillum sp and Aspergillus sp and the growth was found to be decreased with increasing concentration of the extracts

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