Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 08, Issue 1, 2006; Page No.(167-171)

EVALUATION OF SOME FOREST TREE SEED SAMPLES FOR MYCOFLORA AND THEIR MANAGEMENT IN VITRO

M. MAHENDRA, S. LOKESH AND R. RAVISHANKAR RAI

Abstract

In the present study, in order to evaluate the effect of different fungal species on nursery seedlings, seeds of different forest tree species were screened in the laboratory for the occurrence of fungi. Seed samples of different forest tree species like Gmelina arborea, Bauhinea variegata, Dalbergia latifolia and Hardwikia bipinnata were screened for the occurrence of fungal species. Seeds treated with hot water at 500C: showed enhancement in the percentage of germination. Seeds treated with 0.2% Bavistin showed maximum reduction in the incidence of fungi, there by enhanced the germination to a greater extent. In the seed samples, different fungi like Aspergillus niger, A. flavus, A. flavus-oryzae, A. columnaris, A. ochraceus, Penicillium spp. Fusarium moniliforme, F. solani and F. oxysporum were recorded at variout levels. Leaf extracts of Azadirachta indica, Clerodendron inerme, Tridax procumbens and Plumbago zeylanica showed little effect on mycoflora. Comparatively, the best results were obtained with the seeds treated with 0.2% Bavistin. Seedlings of Gmelina arborea and Bauhinea variegata raised in nursery beds showed high incidence of leaf blight. Powdery mildew in Bauhinea varigata and Fusarium wilt in Hard wickia bipinnata were also noticed at high incidence. In Dalbergia latifolia dead seedlings showed high incidence of Aspergillus flavus-oryzae.

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