CHEMOPROTECTIVE EFFECT OF EUGENOL ON CHLORPYRIFOSINDUCED TOXICITY IN RAT ERYTHROCYTESROOPALI PANWAR, SAVITA PRASHAR, RAVI KANT AND RAVI KIRANAbstract The present study was carried out to study the chemoprotective effect of Eugenol on chlorpyrifos induced toxicity in rat erythrocytes. To carry out the investigations, female wistar rats weighing about 100-150 g were randomly segregated into four groups: control, chlorpyrifos, eugenol, chlorpyrifos+eugenol treated. After fifteen days of dozing (chlorpyrifos 89.4mg/kg B.W, eugenol 250 mg/ kg B.W) blood was taken and erythrocytes were separated for various analysis. Chlorpyrifos treatment resulted in statistically significant decrease in activity of G6PD in erythrocytes which was significantly improved by eugenol treatment. activities of antioxidant enzymes , superoxide dismutase, catalase and glutathione-S-transferase were found to be significantly high in chlorpyrifos treated animals indicative of adaptive response of animals towards increased oxidative stress , however this was significantly decreased on administration of eugenol along with chlorpyrifos. A decrease in content of total lipid cholesterol and protein in erythrocytes membranes was observed in chlorpyrifos treated animals, which was also responsible for altered morphology of erythrocytes. However co-administration with eugenol normalized the membrane composition of erythrocytes. Therefore, It could be concluded from the study that chlorpyrifos exerts its toxic effects in erythrocytes by increasing the oxidative stress, however the co-administration of eugenol alogwith chlorpyrifos could modulate chlorpyrifos induced toxicity quite efficiently in erythrocytes.
Enter your contact information below to receive full paper.
|