Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 3, 2005; Page No.(383-385)

OPTIMIZATION OF CHITINASE PRODUCTION BY SERRATIA MARCESCENS GG5

GURSHARAN SINGH NEETU DAHIYA AND GURINDER SINGH HOONDAL

Abstract

A chitinase producing Serratia marcescens GG5 was isolated from decomposed stalks of mushroom. Maximum chitinase production (0.8 U/ml) was observed in minimal medium having 2% swollen chitin after 96 hours of incubation at 30°C and 150 rpm. The enzyme activity was detected only when the bacterium was grown in the medium containing chitin or fungal cell wall. Soluble starch (0.5%), arabinose (0.5%) and Mucor rouxi cell wall (0.2%) enhanced the chitinase production by 30%, 12% and 87.5% when used with swollen chitin in production medium.

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