SCREENING BIOCHEMICAL MARKERS FROM MUSCLES AS PMI INDICATORManasi V. Tukrul and Minakshi GuravAbstract Post-mortem interval (PMI), or time since death, is a matter of crucial importance in any death investigation. Recent developments in biochemical technologies have started to identify biomarkers in different biological fluids such as blood, urine and tissues such as muscle, liver, brain for PMI estimation. The present study focusing on the use of muscle in PMI estimation suggest that the muscle was examined biochemically for enzyme like alkaline phosphatase, alanine aminotransferase, aspartate amino transferase, lactic acid, catalase etc. Labeo calbasu fishes were sacrificed for estimating activity of bimolecular markers to determine postmortem changes in their concentration at the intervals of 0, 3, 4.5, 6 and 24 hrs. Enzyme activity levels so obtained were charted and statistically studied and graphical records were obtained against post-mortem interval. Use of these biochemical markers could be promising tools to determine Post-mortem interval. In this study we observed insignificant correlation between the post-mortem concentration of biochemical parameters such as alkaline phosphatase, alanine aminotransferase, aspartate amino transferase, lactic acid, catalase due to effects of certain factors such as age, condition of fish, sex etc. |