Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 2, 2005; Page No.(319-322)

SCREENING OF MATRICES FOR THE IMMOBILIZATION OF YEAST CELLS FOR LACTOSE HYDROLYSIS

REEBA PANESAR', R.S. SINGH, P.S. PANESAR. AND M.M.B. BERA

Abstract

The present investigation deals with the screening of different immobilizabon matrices to develop a suitable immobilized cell system for the lactose hydrolysis. The different immobilization matrices such as agarose, carrageenan, agarmgar and alginate were used for immobili.tion of perrneabilized yeast net Is for their use in the lactose hydrolysis. The yeast cells immobilized M different matrices showed varied degree of hydrolysis. Out of these tested matrices, alginate entrapped cells showed maximum lactose hydrolysis. The maximum lactose hydrolysis was observed with 2% holy) alginate concentration after 50 min of incubation period.

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