Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 2, 2005; Page No.(315-318)

INFLUENCE OF HEAVY METALS ON GROWTH AND LACCASE PRODUCTION IN PHANEROCHAETE CHRYSOSPORIUM NCIM 1197

M. JAYAPRAKASH VEL.SAHAYAFRAVIN, A. GNANAMAN, MARY BABU AND S. SADULLA

Abstract

A Laccase producing strain Phanerochade clnysospon urn NCIM 1197 ' Copper in the form of copper sulfate at different concentrations ranging from 0.01 inM to 0.5 niM increa'ses' laccam activity substantially. Maximum laccase activity of about 26 U/L was observed with 0.3 n8A CuS0,, Moreover, manganese in the form of MrSO4 in growth medium plays a significant role in enzyme production. Absence of manganese resulted in high yield and activity of las-ram Regarding growth, 03 mL1 Cu concentration resulted in an nitrated mycelial fresh weight of about 770 mg on day 12. It has been suggested that growth medium w ith 0.3 inhA Cu and zero manganese concentration increases both growth and laccase activity in Phanerothuele cluysosporium NCIM 1197.

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