Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 07, Issue 2, 2005; Page No.(265-268)

IN-VITRO PROPAGATION OF LAUNAEA NUDICAULIS, A MEDICINALLY IMPORTANT HERB, THROUGH CALLUS CULTURE

BIRENDRA SINGH, IFFAT ZAREEN AHMAD AND S. SHANTHY

Abstract

Fast growing cultures of Lemma molinnelis can be achieved through callusailture in Moranuge and Skoog medium with 3% sucrose and O.8% agar, good callus growth was observed in MS medium Containing 2.5 ppm 2,440 and 0.1 ppm kinetin. These calli could be maintained in MS medium nf half strength with similar hormonal concentration by regular sub culturing at every 23rd day. Organogenc.is was better in MS medium containing 2.0 ppm IBA and 0.1 ppm IAA. Cultures incubated in dark shmved marked differentiation in comparison to light. Some calli were maintained in MS medium of half strength containing 2.0 ppm IBA and 0.1 ppm IAA after excision of small plantlets, which provides continuoussource of microprogagules. Rooting of the shoots was maximum in MS medium supplemented with 2.0 ppm IBA

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