PRODUCTION OF A THERMOSTABLE PROTEASE BY BACILLUS SP. APR-4DINESH KUMAR, HARISH GAIJU AND TEK CHAND BHALLA
A thermostable protease producing strain identified as Bacillus sp. APR-4 was isolated from cow dung compost sample. Culture conditions were optimised for protease production. Maximum enzyme production was detected in late log phase. Bacillus sp. APR-4 showed maximum production at 50°C, pH 7.0 with 5% inoculum, after 40 h incubation. The optimum temperature and pH for the activity of this protease were 65-70°C and pH 9.0 respectively. Out of the various carbon and nitrogen sources tested, glucose (1%) and yeast extract (0.5%) and peptone (0.5%) proved 10 be very good source/s of carbon and nitrogen respectively for the production of protease by this organism. The enzyme was stable at 50, 65, 70 and 80°C for 5, 5, Ih and 30 min respectively and half life of 150 min at 70°C and 90 min at 80°C. The enzyme was very stable in iso-amyl alcohol (106%) followed by petroleum ether (102%) and ethanol (100%) and was inhibited by EDTA (ethylenediaminetetracetic acid), SDS (sodium dodecyl sulphate) and DTT (dithiothreitol).
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