TRIPLE INOCULATIOIN OF A VAM FUNGUS AND TWO NITROGEN FIXING BACTERIA ON TWO CEREALSK. AMMAN
The effect of combined inoculation of sorghum and pearl millet with nitrogen fixing bacteria, Azospirillum lipoferum, Azotobacter chroococcum and vesicular arbuscular mycorrhizal fungus, Glomus fasciculatum was studied in a pot culture in both sterile and unsterile soil. Different combinations were used in inoculating the plants and the samples were taken after 30, 60 and 90 days of plant growth. The percent colonization by the VAM fungus and the average number of spores was recorded at 90 days. Triple inoculation with all the three microorganisms was better than dual or single inoculation for fungal development and plant growth. Plant response was more pronounced in sterile soil than in unsterile soil. Inoculation with Glomus fasciculat um and the nitrogen fixing bacteria enhanced infection and spore production by Glomus fasciculatum. The dry weights and per cent P and per cent N of both the hosts inoculated with both mycorrhizal and nitrogen fixing bacteria were greater than the control plant suggesting a synergistic interaction between VAM and bacterial fertilizers.
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