Ecology, Environment and Conservation Paper

IN-VITRO EVALUATION OF NOVEL FUNGICIDES AGAINST LEAFSPOT AND FLOWER BLIGHT OF MARIGOLD BY DIFFERENTTECHNIQUES

Rakesh Roshan Satapathy and Kartik Chandra Sahoo

Abstract

Leaf spot and flower blight caused by Alternaria zinniae is serious disease of marigold causing yield loss of50-60 % in crop. Many previously used fungicides showed resistance against the pathogen. So, the presentinvestigation was carried out with an objective to study the efficacy of new fungicides availablein themarket using poison food technique, well diffusion technique and disc diffusion technique. Three fungicidessuch as azoxystrobin +tebuconazole, tebuconazole +sulphur, Metalaxyl were evaluated at the concentrationof 0.1%, 0.15%, 0.2% respectively. The present investigation revealed that Azoxystrobin + Tebuconazolerecorded maximum mycelial growth inhibition of 96.85% followed by Tebuconazole + Sulphur 96.40% andMetalaxyl of 95.50% in poison food technique. Similarly, Azoxystrobin + Tebuconazole showed the highestinhibition of mycelial growth (88.10%), followed by Tebuconazole + Sulphur (86.36%), and Metalaxyl(84.38%) in well diffusion technique. In a same manner Azoxystrobin + Tebuconazole showed the highestinhibition of mycelial growth (77.73%), followed by Tebuconazole + Sulphur (75.10%), andMetalaxyl(72.25%) in disc diffusion method. Further the effectiveness of new fungicides may be tested under fieldcondition against Alternaria zinniae.