Ecology, Environment and Conservation Paper

Vol 29, Issue 1, 2023; Page No.( 1-9)

THE ACTIVITY OF BACILLUS THURINGIENSISVECTOBAC G ONTHE BIOCHEMICAL COMPOSITIONS OF CULEX PIPIENS ANDCULISETA LONGIAREOLATA (DIPTERA: CULICIDAE) MOSQUITOLARVAE

Ali Bouaziz1 Lynda Aissaoui and Hamid Boudjelida

Abstract

Culex pipiens and Culiseta longiareolata, are domestic mosquitoes, and ones of the mostdominant andabundant mosquito species in the urban areas of Algeria. Thus, the present study was conducted to evaluatethe larvicidal activity of Bacillus thuringiensis (Bt.) Vectobac G and to estimate the disturbanceof main bodymetabolites in the tissues of fourth instar larvae of Cx. pipinesand Cs. longiareolata. The toxicologicalessays were done using different concentration of Btivectobac G against a new exuviated fourth instarlarve of Cx. pipiens, and Cs. longiareolata for 24h of exposure time. The results showed a toxic effect witha concentration-response relationship of this product. The biochemical analyses were carried out with theestimated lethal concentrations of both used species (Cx. pipiens: LC50=0.81 g /l andLC90=3.57 g /l; Cs.longiareolata: LC50=1.80 g/l and LC90=5.43 g/l) on the fourth instar larvae. As a result,Bti. Vectobac Gcaused a significant decrease in the whole body weight in the treated larvae as compared to the controlseries. Moreover, biochemical analyses revealed an increase in carbohydrates levels, and a decrease inprotein and lipids in the whole larvae body treated with the lethal concentrations as comparedto thecontrol series for the both species Cx. pipiens and Cs. longiareolata.