Ecology, Environment and Conservation Paper

Vol. 29, Jan. Suppl. Issue 2023; Page No.(364-368)

IN VITRO DEGRADATION OF PROFENOFOS BY RICE GRAIN RINSEWATER LACTIC ACID BACTERIA (LAB)

A. Subash Chandra Bose, P. Yasodha, K.G. Sabarinathan and P. M. M. David

Abstract

Widely used in fermentation industry, lactic acid bacteria (LAB) are not only probiotic but also capable ofdegrading pesticides. We evaluated in the laboratory degradation of profenofos 50% EC by the LAB presentin rice grain rinse water cultured in LAB-specific de Mann Rogosa Sharpe (MRS) medium. A single colonyof the isolated LAB was inoculated in MRS broth, kept in shaker for 24 h before 10 ml broth was transferredto 25 ml test tube inoculated with profenofos 50% EC at 1 ppm and checked 24, 48 and 72 h after inoculationfor its residue by liquid-liquid extraction and gas chromatographic analysis. The results revealed thatprofenofos concentration decreased to 0.75, 0.44 and 0.25 ppm at 24, 48 and 72h, respectively, indicating thedegradation of profenofos by the rice rinse water LAB.