ISOLATION, SCREENING AND OPTIMIZATION FOR LACCASEPRODUCTION BY FUSARIUM SPP. UNDER SUBMERGEDFERMENTATIONThirthalakshmi Yochitha S., Soundarya Shree K.R., Bhavana D.1, Arpitha H.,Harsha T.S. and Nagalambika PrasadAbstract Laccases are oxidases with broad substrate specificity and ability to oxidize various phenolic and nonphenoliccompounds. This study was carried out to isolate and characterizes laccase producing fungi fromthe environmental samples. Soil samples were collected from different locations. Suspensions of the samples(1g in 10 ml sterile physiological saline) were serially diluted, inoculated onto Czapek Dox Agar (CZA)containing antibiotic chloramphenicol and incubated for 7 days at 30 oC. The fungal isolates werecharacterized macroscopically and microscopically. Screening was done using Czapek-Dox agar wassupplemented with 0.1% tannic acid, 0.5% Bromophenol blue, 0.05 ml in 25 ml of Methanol Guaiacol thepure culture of the single colonies from the spread plate method were inoculated on the media and incubatedat room temperature for 5-6 days. After 5-6 days the plates were checked for clear transparent zone. |