Ecology, Environment and Conservation Paper

DETECTION OF AZOXYSTROBIN IN ENVIRONMENTAL SAMPLESUSING FTIR SPECTROSCOPIC METHOD

Ajay Kumar Sahu, Bharat Lal Sahu, Shobhana Ramteke, Yaman Kumar Sahu,Manoj Kumar Banjare, Manish Kumar Rai and Joyce Rai

Abstract

A new UV-Visible spectrophotometric method for determination of fungicide azoxysrtobin was developed.The method is based on the bromination of azoxystrobin to form dibromoazoxystrobin which react withPotassium iodide, Potassium iodate mixture in the presence of leucomalachite green (LMG) to form a bluishgreen colored complex. Characterization was done for the synthesis of bluish green colored complex byusing UV-Vis spectrophotometer and FTIR methods. As a result, the UV-Visible absorption spectrum wasobserved at 615 nm. The limits of detection and limits of quantification were observed at 0.0019 μg ml-1 and0.0059 μg ml-1 respectively. We have also studied the conformational and functional group (such as mostcharacteristic band of O-H stretching frequency observed at 3346.49 cm-1, Bonding N-H symmetrical is1645.50 cm-1, C=C bending is 691.07 cm-1, Symmetrical stretching C-N is 1493.13 cm-1 and 1404.70 cm-1, C-Cstretching and other vibrational is 1059.74 cm-1). Involved in the complexation between azoxystrobin andbromination by FTIR method. This developed method has been successfully applied for the detection ofazoxystrobin in various environmental samples. Beer’s law obeyed over the concentration range of 0.5-13μg ml-1 in final solution volume of 10 ml. The reproducibility assessed by carrying out seven days replicateanalysis of a solution containing 5 μg ml-1 of azoxystrobin in a final solution of 10 ml. The molar absorptivityof the colour system is 1.936×106 L mol-1 cm-1 and Sandell’s sensitivity is 0.800 ×10-4 μg cm-2. The relativestandard deviation (RSD) for the absorbance value was found to be 1.9%. The suggested method is freefrom the interference of other toxicant agents. The analytical parameters were optimized and the methodwas applied to the determination of azoxystrobin in water, soil and food samples.