ENDOCRINE DISRUPTION AND PHYSIOLOGICAL ALTERATION INFISH EXPOSED TO HEXAVALENT CHROMIUMRashmita Behera, Bidut Prava Mohanty, Malaya Ranjan Mahananda and Samikshya MishraAbstract Sensitivity detection of hexavalent chromium in Tilapia fish (Oreochromis mossambica) during toxicity testshows the physiological alteration and endocrine disruption. Biochemical impairments of Hexavalent chromium at various sublethal concentrations, i.e., 10 ppm, 30 ppm, 60 ppm, and control for 24 hr, 48 hr, 72hr, and 96 hr in common freshwater fish Oreochromis mossambica (Tilapia fish) was monitored during thediverse exposure periods. The blood sample was collected, and the ALP test was performed byspectrophotometric technique to observe cytotoxic or genotoxic effects. Behavioral and morphologicalalterations were elucidated in the course of the investigation. Muscle, skin, a gill of fish were collected toestimate total protein and carbohydrate to notice the growth and development pattern. Toxicity tests weredetermined with probit analysis in SPSS software. The maximum increase in the ALP content was 189.47%at 96 hr of exposure at 60 ppm of hexavalent chromium. The maximum decrease in carbohydrate content ofmuscle, gill, and skin was observed to be 43.33%, -50%, and -48.27%, respectively, and protein content as -64.93%, -44.44%, and -66.67%, respectively, at 96 hr LC50 of exposure at 60 ppm of hexavalent chromium.LC50 values indicated that Cr 6+ was highly toxic in muscle, gill, and skin of Oreochromis mossambica asthere was a drastic reduction of biochemical parameters (carbohydrate, protein). The increase in ALP contentin male fish due to Chromium concentration revealed that Cr 6+ acts as a potent endocrine disruptor. Hencethe male fish was selected to find the precursor protein vitellogenin as a standard exposure indicator. |