Ecology, Environment and Conservation Paper

Vol 28, Issue 3, 2022; Page No.(1135-1138)

DIFFERENT CONCENTRATION ORGANIC CARBON WASTECASSAVA IN MIXOTROPHIC CULTIVATION ON PROTEIN ANDBETA CAROTENE CONTENT OF DUNALIELLA SP.

Prive Widya Antika1, Happy Nursyam and Arning Wilujeng Ekawati

Abstract

Dunaliella sp. is one type of green microalgae that can be used as natural feed because it is easy to digest. Inmixotrophic culture, microalgae in their growth carry outthe process of photosynthesis and use organiccarbon nutrients to produce energy. Choice of organiccarbon sources for growing Dunaliella sp. namelyusing waste cassava. The organic carbon content in wastecassava is still very complex, so that Dunaliella sp.for its growth, it can be done by breaking downcarbohydrates into simple glucose using hydrolysis withthe addition of -amylase and glucoamylase enzymes. The purpose of this study was to explain the effectof giving waste cassava hydrolyzate under Mixotrophic conditions to the protein and beta carotene contentof Dunaliella sp. The results showed that the administration of glucose organic carbon from different wastecassava under mixotrophic conditions had a significanteffect on the protein and beta carotene content ofDunaliella sp. The best glucose concentration of 0.30 g/lresulted in 34.69% protein content and 19.84 g/mlbeta carotene.