EFFICACY OF RAUVOLFIA TETRAPHYLLA LEAF EXTRACTS AGAINSTTHE VECTOR OF LYMPHATIC FILARIASISCULEX QUINQUEFASCIATUSRadhakrishna S. Pandit and Kishor D RautAbstract The Rauvolfia tetraphylla leaf extract with different solvents n-hexane, chloroform, acetone and methanolwere tested for larvicidal, ovicidal, oviposition attractant/deterrent activities. Their effect on larval nutritionalreserve against Culex quinquefasciatus was also studied. Larval mortality was observed after 24 hoursexposure. The LC50 values for n-hexane, chloroform, acetone, methanol extracts are 16.80, 91.56, 214.85,44.68 ppm, respectively. The mean percentage hatchability of the ovicidal activity was observed 48 hourspost treatment. For R. tetraphylla, methanolic extract exhibited 56 % egg hatchability at 125 ppm. The effectiveoviposition repellency was 100 % at 320 ppm and the oviposition activity index value -1 was shown by nhexaneextract of R. tetraphylla. The fourth instar of C. quinquefasciatuswhere exposed to LC50 concentrationof extracts and its on larval nutritional reserves was checked. All extracts showed moderate effect on larvalnutritional reserves, i.e. changes in total concentration of sugar, glycogen, lipid and protein. The averagevalues of total sugar, glycogen, lipid and protein are 9.47, 32.32, 54.50, 183.35 μg/larvae was found infourth instar larvae of C. quinquefasciatus. From the all-leaf extracts, the methanol extract affects the totalsugar, lipid; n-hexane extract to glycogen and acetone extract to protein of fourth instar larvae of the mosquitoand decreases up to 3.39, 20.71, 11.18, 91.05 μg/larvae, respectively. These results reveal that the R. tetraphyllaleaf extracts have the control potential against C. quinquefasciatus. |