BIOCHEMICAL CHANGES INDUCED BY FLUBENDIAMIDE (39.35%SC) IN FRESHWATER FISH, CATLA CATLA (HAMILTON, 1822)SK. PARVEEN, K. VEERAIAH², G. VANI, M.VIJAYA KUMAR AND CH. KRISHNAAbstract The fish Catla catla fingerlings were exposed to the test toxicant Flubendiamide, a new lepidopteron insecticide, used to control important lepidopteron pests. The acute toxicity tests for24, 48, 72 and 96 h were conducted and the LC50 values were calculated using Finneys probitan alysis and were reported to be 3.566 mg.l-1, 3.456 mg.L-1, 3.0221 mg.l-1 and 2.892 mg.l-1, respectively for 24, 48, 72 and 96 h. After determination of LC50values the fingerlings were exposed to sub-lethal(0.289 mg.l-1) and lethal (2.892 mg.l-1) concentrations of Flubendiamide for 24, 48, and, 96 h, and 8days exposure was made only to the sub-lethal concentration to study the alterations in different biochemical parameters viz, total glycogen, total proteins, and the changes in the contents of the nucleic acids such as DNA and RNA. The changes in the biochemical constituents of the vitalorgans viz, Gill, Liver, Brain, Muscle, and Kidney were studied. Significant changes in the biochemical constituents of these organs of fish were observed. All the biochemical constituents were found to be decreased in different tissues and the results obtained in all were discussed in the light of metabolic stress caused due to the exposure of the toxicant. |