Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

Vol 24, Issue 2, 2022; Page No.(314-321)

BACTERIAL TREATMENT OF MELANOIDIN CONTAINING POSTMETHANATED DISTILLERY SPENT WASH

PRITI MAHLA AND NIKHIL BHATT

Abstract

Untreated or primary treated distillery spent wash is lethal for the environment due to its highlyacidic and other physicochemical impurities. An attempt to screen out potent bacterial consortium formelanoid in containing post methanated distillery spent wash (PMDSW) was carried out. Developedbacterial consortium was subjected to treat PMDSW for decolorization and degradation purpose. BBDdesign was projected and achieved significant variable was founded as 0.2% urea concentration, 20%PMDSW concentration and 40 h incubation in static with 99.99% decolorization. Enzymes involved in thedegradation process (manganese peroxidase, lignin peroxidase and laccase) was assayed as well as analyzedwith HPTLC and FTIR techniques for confirmation of degradation as reduction in peak height and area.Toxicity removal was also being proceed as phytotoxicity with Vigna radiata and achieved 95% seedgermination and measured other plant growth parameters. The consortium was isolated (3.2022) on solidmedium and identified with 16S rRNA sequencing.