Asian Journal of Microbiology, Biotechnology & Environmental Sciences Paper

COMPARATIVE VALIDATION OF SELF COLLECTED AND MARKET‘SARIVA’ ROOTS WITH RESPECT TO THEIR AMBIGUITY IN THEIDENTIFICATION USING IN-VITRO BIOCHEMICAL ASSAYS

EITISHA SANDECHA, NINAD NANGARE, ANURADHA MULIK,SURESH JAGTAP AND NEELAMBIKA METI

Abstract

Plant ‘Sariva’ has been used since long in Ayurveda and folk medicine. There are four differentspecies used as ‘Sariva’ in various regions of India viz., Cryptolepisbuchanani Roem. & Schult., Hemidesmusindicus (L.) R.Br. and Decalepishamiltonii Wight & Arn belongs to same family Asclepiadaceae, whileIchnocarpus frutescens (L.) R. Br. belongs to family Apocynaceae. Present study is designed to evaluate theambiguity in market and authenticate samples of ‘Sariva’ root extract in terms of their polyphenolic contentsand antioxidant profile. Market samples were collected from major markets from India viz., Banglore, Delhi,Mumbai, Nagpur, Pune and West Bengal. While, authenticate species of ‘Sariva’ was collected from WesternGhats of Maharashtra, India. Antioxidant profile was evaluated by nitric oxide scavenging potential, BSAanti-denaturation assay and anti-lipid peroxidation assays. Total phenolic and flavonoid contents werestudied by standard methods viz. Folin-Ciocalteu and aluminium chloride respectively. Methanolic extractof ‘Sariva’ market sample showed highest phenolic contents (0.295±0.023μg/ml) as compared to theflavonoid contents in Pune market sample. While, highest concentration of total phenol contents wasobserved in Decalepishamiltonii (0.346±0.034μg/ml) and flavonoids was observed in Cryptolepisbuchanani(0.11±0.007mg/ml). The methanolic extract of West Bengal showed highest anti-lipid peroxidation activity(IC50 value of 50.96μg/ml). While, lowest activity was observed in Delhi market sample (IC50 of 218.63). Onthe contrary, Hemidesmus indicus showed highest anti-lipid activity (28.88 μg/mL of IC50) followed byDecalepishamiltonii, Cryptolepisbuchanani and Ichnocarpus frutescent.IC50 value indicates that among all themarket samples Banglore market sample (67.26 μg/ml) showed more significant protein stabilizing activity(denaturation is inhibited). While, Decalepishamiltonii showed more significant protein stabilizing activity(32.73 μg/ml) followed by Hemidesmus indicus, Cryptolepisbuchanani and Ichnocarpus frutescent. Amongmarket samples, Pune market sample showed highest nitric oxide scavenging activity (65.15μg/ml of IC50)and in authenticate samples, Decalepishamiltonii showed highest nitric oxide scavenging potential (31.26 μg/mlof IC50). By evaluating the five market samples in comparison with authenticate ‘Sariva’ species, it isconcluded that distinct variation observed in polyphenolic contents and antioxidant activity. Hence, presentstudy suggests the need of awareness in the selection of the correct species and scientific efforts to solve thisambiguity.