Ecology, Environment and Conservation Paper

MOLECULAR CHARACTERIZATION OF VIRUS SPECIES CAUSINGYELLOW MOSAIC DISEASE OF REDGRAM IN ANDHRA PRADESH

B.H. Chaithanya, B.V. Bhaskara Reddy, L. Prasanthi and R. Sarada Jayalakshmi Devi

Abstract

Redgram is animportant drought tolerant leguminous crop in the semi-arid subtropics. Many fungal, bacterialand viral diseases are major constraints for redgram production, apart from all these diseases, yellowmosaic disease (YMD) is observed in redgram crop in few localities of Chittoor district, Andhra Pradeshduring 2017. The present study was aimed to find out associated begomo virus species causing YMD inredgram.The total genomic DNA was isolated from YMV infected redgram leaves by modified CTAB method.The rolling circle amplification based full length clones were constructed in pUC18 vector. The two constructed clones (DNA-A and DNA-B) were confirmed by using gene specific primers and weresequenced.The coat protein gene andcomplete nucleotide sequence analysis was carried out with the knownbegomoviruses sequences obtained from Genbank database.The phylogenetic analysis of coat protein geneof YMV-AP-Red gram isolate shared 94.3-98.1% nucleotide identity with HgYMV isolates, followed byMYMV (81.2-85.2%), MYMIV (78.1-80.2%) and DoYMV(72.2-74%). Hence it was confirmed that viral speciescausing YMD in redgram from Tirupati, AndhraPradesh is a Horsegram yellow mosaic virus and it is avariant of HgYMV as it shared>94% similarity with other HgYMV isolates at nucleotide level.