Ecology, Environment and Conservation Paper

Vol 28, Issue 1, 2022; Page No.(530-534)

ANTIBACTERIAL ACTIVITY TEST OF MIXTURE SEED EXTRACTS OF MORINGA OLEIFERA LAMK AND SCHLEICHERA OLEOSA L. AGAINST STAPHYLOCOCCUS EPIDERMIDIS AND SHIGELLA DYSENTERIAE

Anggreini D.N. Rupidara, Melkhior M. Seran and Mellissa E.S. Ledo

Abstract

Moringa (M. oleifera Lamk.) is known to have antibacterial ability, because the seeds contain tannin, flavonoids, alkaloids, and saponins. Kesambi or Malay lac tree (S. oleosa L.) has been used to fight skin inflammation, ulcers, itching, acne and skin infections. Its seeds contain oil, which is very good for the skin. The ability of a mixture of the seed of moringa and kesambi has not been widely reported. This study aims to determine the inhibitory power resulting from a mixture seed extracts of Moringa and kesambi against S. epidermidis and S. dysenteriae. The method used is experimental using CDR (Completely Randomized Design) with 5 treatments and 3 replicates which are P1 = 10 g Moringa + 0 g Kesambi, P2 = 2.5 g Moringa + 7.5 g Kesambi, P3 = 5 g Moringa + 5 g Kesambi, P4 = 7.5 g Moringa + 2.5 g Kesambi, P5 = 0 g Moringa + 10 g Kesambi. One way Anova and Tukey test were performed for statistical justification. The inhibitory test of the mixture seeds extract of moringa and kesambi showed that there was antibacterial activity against S. epidermidis and S. dysenteriae. The mixed extract showed the maximum effect on Gram (+) S. epidermidis, namely P3 with inhibitory power of 25.33±0.76 mm. While on the Gram (-) bacteria, S. Dysenteriae, the P4 had the highest inhibition (9.17±1.15 mm). The mixture of Moringa seed extract and kesambi seed has a higher effect on gram positive bacteria.