EXTRACTION OF BIOACTIVE COMPOUNDS IN GANODERMA LUCIDIUM FROM HIMACHAL PRADESHK. Theresa, Vimalin Hena and Jasmin DoraAbstract Ganoderma lucidium from the region of Himachal Pradesh was taken for the study to identify the bioactive compounds. Two extraction methods viz., water and methanol extract were used for extracting the compounds. From the results, when comparing with water extract of fruiting body of Ganoderma lucidium the methanol extract has high number of phenolic and triterpenoid compounds. Elemental analysis revealed that calcium, sodium phosphorous, potassium was in high concentration. While potassium, magnesium, copper, iron, zinc, manganese were found to be in moderate level. TLC spotted with chromatographic fractions showed pink spots when sprayed with anisaldehyde-sulphuric acid reagent which indicate the presence of triterpenes. In HPLC chromatogram of methanolic extract of Ganoderma lucidium eight compounds were detected and these compounds were extracted at retention time 4.87, 21.67, 26.2, 29.1, 30.09, 36, 38.2, 39 respectively. From GC-MS chromatograms, 13 compounds were identified mostly these compounds were saturated and unsaturated fatty acid. These compounds were analysed for pharmacological study. |