Ecology, Environment and Conservation Paper

EFFECT OF POLYPHENOL FRACTION EXPOSURE OF ALOE VERA ON THE CHANGE IN CORTISOL OF KOI FISH (CYPRINUS CARPIO) AS STRESS RESPONSE

Sri Andayani, M Sulaiman Dadiono, Widya Tri Elwira, Husna Nabila, Rodhyansyah, Nelvan Subayu and Ashari Fahrurrozi

Abstract

This study aims to analyze the stress response of koi fish exposed to polyphenol fraction of A. vera, metabolic effect of cortisol. It firstly found LC50 at the treatment of 150 mg.kg-1 that caused 50% mortality, so that the doses of A. vera fraction injected into the fish muscle as follows: Control (-) no treatment, Control (+) was given tannin/synthetic polyphenolic compound as much as 3 mg.kg-1 of body weight, treatment A=75 mg.kg-1 of body weight, B= 100 mg.kg-1of body weight, C- 125 mg.kg-1 of body weight, respectively. After 72 h of injection, the blood plasm of Koi fish was taken to observe the stress effect on the cortisol. Results showed that for cortisol rose with increased polyphenol fraction application due to higher stress level.